The Effects of Sex Steroids on the Maxi-K Channel Activity of Human Vas Deferens / 대한비뇨기과학회지
Korean Journal of Urology
;
: 867-872, 2005.
Article
in Korean
| WPRIM
| ID: wpr-196363
ABSTRACT
Purpose:
We investigated the effects of sex steroids on the maxi-K channel activities of the single smooth muscle cells of the human vas deferens. Materials andMethods:
Human vas deferens cells were isolated using proteolytic enzymes (collagense and papain), and then they were employed for an electrophysiological study with using the inside-out patch clamp configurations. The intracellular calcium concentrations were adjusted between 0.1mum to 1mum, and the membrane potentials were depolarized.Results:
The maxi-K channels were identified by their single channel conductance and calcium dependency. The application of 10ng/dl estrogen induced a significant increment of the maxi-K channel activities. The effects were not affected by the change of the intracellular calcium concentration. The other sex steroids (progesterone and testosterone) rarely affected the maxi-K channel activities.Conclusions:
Estrogen has stimulatory effects on the maxi-K channel activity of human vas deferens. This suggests that estrogen may relax the human vas deferens via maxi-K channel activation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Hydrolases
/
Steroids
/
Vas Deferens
/
Calcium
/
Myocytes, Smooth Muscle
/
Estrogens
/
Large-Conductance Calcium-Activated Potassium Channels
/
Membrane Potentials
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2005
Type:
Article
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