Down-regulation of human FEN-1 gene expression during differentiation of promyelocytic leukemia cells
Experimental & Molecular Medicine
;
: 252-256, 1998.
Article
in English
| WPRIM
| ID: wpr-196909
ABSTRACT
Flap endo/exonuclease-1 (FEN-1) recognizes 5'-flap DNA structures that have been proposed to be important intermediates in DNA replication, repair and recombination, and cleaves the double strand-single strand junction of flap substrates. Using an in vitro model system, recent studies have shown that FEN-1 is a necessary enzyme for the removal of RNA primers in Okazaki fragment maturation during lagging strand DNA synthesis. In this report, the FEN-1 gene expression was examined during cell cycle and differentiation. Although FEN-1 mRNA and protein could be detected at all stages of the cell cycle, their levels were more elevated in exponentially proliferating cells than in G1 or G2/M-synchronized cells. Moreover, a significant increase of FEN-1 protein was observed when temporarily quiescent fibroblasts were induced to proliferate by serum stimulation. In contrast, the FEN-1 mRNA level showed a sharp decrease in HL-60 cells differentiated by dimethyl-sulfoxide, all-trans retinoic acid or 12-O-tetradecanoylphorbol-13-acetate. These results demonstrate that the FEN-1 gene expression is up-regulated during entrance into the mitotic cell cycle and down-regulated in nongrowing cells, as in the case of differentiated promyelocytic leukemia cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia, Promyelocytic, Acute
/
Down-Regulation
/
Gene Expression Regulation, Neoplastic
/
Cell Cycle
/
Cell Differentiation
/
Cell Division
/
Dimethyl Sulfoxide
/
Blotting, Western
/
3T3 Cells
/
HL-60 Cells
Limits:
Animals
/
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1998
Type:
Article
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