Expression of E-cadherin in pig kidney
Journal of Veterinary Science
;
: 381-386, 2013.
Article
in English
| WPRIM
| ID: wpr-197116
ABSTRACT
E-cadherin is a cell adhesion molecule that plays an important role in maintaining renal epithelial polarity and integrity. The purpose of this study was to determine the exact cellular localization of E-cadherin in pig kidney. Kidney tissues from pigs were processed for light and electron microscopy immunocytochemistry, and immunoblot analysis. E-cadhedrin bands of the same size were detected by immunoblot of samples from rat and pig kidneys. In pig kidney, strong E-cadherin expression was observed in the basolateral plasma membrane of the tubular epithelial cells. E-cadherin immunolabeling was not detected in glomeruli or blood vessels of pig kidney. Double-labeling results demonstrated that E-cadherin was expressed in the calbindin D28k-positive distal convoluted tubule and H(+)-ATPase-positive collecting duct, but not in the aquaporin 1-positive, N-cadherin-positive proximal tubule. In contrast to rat, E-cadherin immunoreactivity was not expressed at detectable levels in the Tamm-Horsfall protein-positive thick ascending limb of pig kidney. Immunoelectron microscopy confirmed that E-cadherin was localized in both the lateral membranes and basal infoldings of the collecting duct. These results suggest that E-cadherin may be a critical adhesion molecule in the distal convoluted tubule and collecting duct cells of pig kidney.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cadherins
/
Cell Membrane
/
Gene Expression Regulation
/
Blotting, Western
/
Sus scrofa
/
Microscopy, Electron, Transmission
/
Kidney
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article
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