ERG Immunohistochemistry as an Endothelial Marker for Assessing Lymphovascular Invasion
Korean Journal of Pathology
;
: 355-364, 2013.
Article
in English
| WPRIM
| ID: wpr-19723
ABSTRACT
BACKGROUND:
ERG, a member of the ETS family of transcription factors, is a highly specific endothelial marker. We investigated whether the use of ERG immunostaining can help pathologists detect lymphovascular invasion (LVI) and decrease interobserver variability in LVI diagnosis.METHODS:
Fifteen cases of surgically resected colorectal cancers with hepatic metastasis were selected and the most representative sections for LVI detection were immunostained with ERG, CD31, and D2-40. Eight pathologists independently evaluated LVI status on hematoxylin and eosin (H&E) and the corresponding immunostained sections and then convened for a consensus meeting. The results were analyzed by kappa (kappa) statistics.RESULTS:
The average rate of LVI positivity was observed in 43% with H&E only, 10% with CD31, 29% with D2-40, and 16% with ERG. Agreement among pathologists was fair for H&E only (kappa=0.27), D2-40 (kappa=0.21), ERG (kappa=0.23), and was moderate for CD31 (kappa=0.55). Consensus revealed that ERG nuclear immunoreactivity showed better visual contrast of LVI detection than the other staining, with improved agreement and LVI detection rate (kappa=0.65, LVI positivity rate 80%).CONCLUSIONS:
The present study demonstrated a superiority with ERG immunostaining and indicated that ERG is a promising panendothelial marker that might help pathologists increase LVI detection and decrease interobserver variability in LVI diagnosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
Immunohistochemistry
/
Colorectal Neoplasms
/
Observer Variation
/
Eosine Yellowish-(YS)
/
Consensus
/
Hematoxylin
/
Neoplasm Metastasis
Type of study:
Practice guideline
Limits:
Humans
Language:
English
Journal:
Korean Journal of Pathology
Year:
2013
Type:
Article
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