Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in A549 cells
Journal of Veterinary Science
;
: 321-326, 2006.
Article
in English
| WPRIM
| ID: wpr-197261
ABSTRACT
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endosomes
/
Sucrose
/
Temperature
/
Biocompatible Materials
/
Drug Delivery Systems
/
Microscopy, Confocal
/
Macrolides
/
Sodium Azide
/
Cell Line, Tumor
/
Microscopy, Electron, Transmission
Limits:
Humans
Language:
English
Journal:
Journal of Veterinary Science
Year:
2006
Type:
Article
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