Identification of IgE binding components of two major weed pollens, ragweed and mugwort
Allergy, Asthma & Respiratory Disease
;
: 337-343, 2014.
Article
in Korean
| WPRIM
| ID: wpr-197352
ABSTRACT
PURPOSE:
Ragweed and mugwort pollens are the major weed allergens that cause pollinosis in Korea. The IgE-binding components to these 2 pollens and their cross-reactivity have not been reported in Korea, while several reports had been made in Western countries. We investigated IgE-binding components to ragweed and mugwort pollens and their allergenic relationship in patients sensitive to the 2 pollens.METHODS:
We enrolled 33 allergic rhinitis patients with typical seasonal pollinosis symptoms in autumn and elevated serum specific IgE levels to ragweed and/or mugwort pollens (>10 kU/L by ImmunoCAP). The protein bands of the 2 pollen extracts were determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE immunoblot analysis was performed to determine the IgE-binding components of each pollen extract. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot inhibition tests were performed to evaluate the cross-reactivity between ragweed and mugwort pollen extracts.RESULTS:
Eight IgE-binding components (9, 10, 11, 12, 27, 30, 38, and 80 kDa) were found in ragweed pollen extracts, of which 4 (38, 11, 27, and 80 kDa) were major IgE-binding components. Eight IgE-binding components (10, 14, 16, 20-24, 26-30, 42, 60-66, and 80-90 kDa) were found in mugwort pollen extracts, of which 2 components (26-30 and 20-24 kDa) were major IgE-binding components. No significant inhibitions were noted between ragweed and mugwort pollen extracts by the ELISA inhibition test. No significant changes were noted in IgE immunoblot inhibition analysis.CONCLUSION:
We identified 4 major IgE-binding components (38, 11, 35, 27, and 80 kDa) in ragweed pollens and 2 major IgE-binding components (26-30 and 20-24 kDa) in mugwort pollens. No cross-reactivity was found between ragweed and mugwort pollens.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pollen
/
Seasons
/
Sodium
/
Immunoglobulin E
/
Enzyme-Linked Immunosorbent Assay
/
Allergens
/
Rhinitis
/
Rhinitis, Allergic, Seasonal
/
Artemisia
/
Cross Reactions
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
Korean
Journal:
Allergy, Asthma & Respiratory Disease
Year:
2014
Type:
Article
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