Inhibitory Effect of Apocynin on Proteasome Inhibition-Induced Apoptosis in Differentiated PC12 Cells
Journal of the Korean Neurological Association
;
: 116-123, 2016.
Article
in Korean
| WPRIM
| ID: wpr-197551
ABSTRACT
BACKGROUND:
The dysfunction of the proteasome system has been implicated in neuronal degeneration. Apocynin, a specific inhibitor for nicotinamide adenine dinucleotide phosphate oxidase, has anti-inflammatory and anti-oxidant effects. However, the effect of apocynin on the neuronal cell death induced by proteasome inhibition has not been studied.METHODS:
Using differentiated PC12 cells, in the respect of cell death process the suppressive effect of apocynin on the proteasome inhibition-mediated apoptosis was examined.RESULTS:
The proteasome inhibitors MG132 and MG115 induced a decrease in Bid and Bcl-2 protein levels, an increase in Bax and p53 levels, mitochondrial depolarization, efflux of cytochrome c into cytosol and increase in caspases (-8, -9 and -3) activities. Treatment with apocynin attenuated the proteasome inhibitor-induced changes in the apoptosis-related protein levels, formation of reactive oxygen species, glutathione (GSH) depletion and cell death.CONCLUSIONS:
Apocynin may attenuate the proteasome inhibitor-mediated apoptosis in differentiated PC12 cells by inhibiting the activation of the mitochondria-mediated pathway and the caspase-8- and Bid-dependent pathways. The preventive effect of apocynin appears to be attributed to inhibition of the production of reactive oxygen species and the depletion of cellular GSH contents.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidoreductases
/
PC12 Cells
/
Cell Death
/
Reactive Oxygen Species
/
Apoptosis
/
Caspases
/
Cytosol
/
Cytochromes c
/
Proteasome Endopeptidase Complex
/
Proteasome Inhibitors
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Neurological Association
Year:
2016
Type:
Article
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