Optimization of culture media of pathogenic Mycoplasma hyopneumoniae by a response surface methodology
Journal of Veterinary Science
;
: 327-332, 2010.
Article
in English
| WPRIM
| ID: wpr-197695
ABSTRACT
Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a 'one factor at a time' technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central composite design (CCD) of RSM. The experimental results were fitted into a second order polynomial model equation. Estimated optimal condition of the factors for maximum production of mycoplasmal protein (i.e., triple-fold increase from 0.8 mg/L produced by basal mycoplasma media to 2.5 mg/L) was 10.9% fresh yeast extract, 15% horse serum, and 31.5% porcine serum (v/v). For the optimized conditions, a 2.96 mg/L experimental result was observed, similar to the estimated optimal conditions result of the CCD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biotechnology
/
Culture Media
/
Mycoplasma hyopneumoniae
Language:
English
Journal:
Journal of Veterinary Science
Year:
2010
Type:
Article
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