Comparison Study of Reticulocyte Enumeration by H*3 RTX(TM), FACScan(TM), and Manual Counting / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 218-229, 1997.
Article
in English
| WPRIM
| ID: wpr-198164
ABSTRACT
BACKGROUND:
Reticulocyte counts provide clinically useful informations and the most widely used method for counting reticulocytes is a manual microscopic procedure. Although manual method is inexpensive and relatively simple to perform, it is labor intensive and imprecise. So, more rapid and more reproducible methods are needed.METHODS:
Reticulocyte counts on 96 blood samples were performed by conventional manual method, H*3 RTX(TM)(Bayer, U.S.A.), and FACScan(TM)(Beckon Dickinson, U.S.A.). The changes of reticulocyte count after storage of samples and certain time intervals after preparation were also examined.RESULTS:
Reticulocyte counts(%) by manual method, H*3 RTX(TM), and FACScanM were 2.05+/-2.16, 1.95+/-2.24 and 2.51+/-1.94, respectively. There was no statistically significant differences between manual counting and H*3 RTX(TM)(P>0.05). However, there were significant differences between H*3 RTX(TM) and FACScan(TM), manual counting and FACScan(TM)(P<0.05). Correlation coefficients of three comparisons were all above 0.920. Statistically not confirmed the reticulocytosis sample showed decreasing tendency of reticuocyte count by H*3 RTX(TM) after storage of the sample and prepared sample and others were relatively stable in H*3 RTX(TM)and FACScan(TM).CONCLUSIONS:
The result of the mean difference between H*3 RTX(TM) and manual counting is statistically insignificant. So, the H*3 RTX(TM)can be used interchangeably with manual counting atter consideration of cost-effectiveness.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reticulocytes
/
Reticulocyte Count
/
Reticulocytosis
Type of study:
Practice guideline
Language:
English
Journal:
Korean Journal of Clinical Pathology
Year:
1997
Type:
Article
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