Enhancement of Antigen-specific Antibody and CD8(+) T Cell Responses by Codelivery of IL-12-encapsulated Microspheres in Protein and Peptide Vaccination
Immune Network
;
: 186-196, 2007.
Article
in English
| WPRIM
| ID: wpr-198232
ABSTRACT
BACKGROUND:
Although IL-12 has been widely accepted to play a central role in the control of pathogen infection, the use of recombinant IL-12 (rIL-12) as a vaccine adjuvant has been known to be ineffective because of its rapid clearance in the body.METHODS:
To investigate the effect of sustained release of IL-12 in vivo in the peptide and protein vaccination models, rIL-12 was encapsulated into poly (DL-lactic-co-glycolic acid) (PLGA).RESULTS:
We found that codelivery of IL-12-encapsulated microspheres (IL-12EM) could dramatically increase not only antibody responses, but also antigen-specific CD4(+) and CD8(+) T cell responses. Enhanced immune responses were shown to be correlated with protective immunity against influenza and respiratory syncytial virus (RSV) virus challenge. Interestingly, the enhancement of CD8(+) T cell response was not detectable when CD4(+) T cell knockout mice were subjected to vaccination, indicating that the enhancement of the CD8(+) T cell response by IL-12EM is dependent on CD4(+) T cell "help".CONCLUSION:
Thus, IL-12EM could be applied as an adjuvant of protein and peptide vaccines to enhance protective immunity against virus infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Respiratory Syncytial Viruses
/
Vaccination
/
Mice, Knockout
/
Interleukin-12
/
Vaccines, Subunit
/
Influenza, Human
/
Microspheres
/
Antibody Formation
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2007
Type:
Article
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