From rabbit antibody repertoires to rabbit monoclonal antibodies
Experimental & Molecular Medicine
;
: e305-2017.
Article
in English
| WPRIM
| ID: wpr-198937
ABSTRACT
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacteriophages
/
B-Lymphocytes
/
Sensitivity and Specificity
/
Hybridomas
/
Indicators and Reagents
/
Antibodies, Monoclonal
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2017
Type:
Article
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