Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs
The Korean Journal of Parasitology
;
: 59-64, 2002.
Article
in English
| WPRIM
| ID: wpr-19910
ABSTRACT
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Cattle
/
Antibodies, Protozoan
/
Molecular Sequence Data
/
Base Sequence
/
Protozoan Proteins
/
Zinc Fingers
/
Amino Acid Sequence
/
Cryptosporidium parvum
/
Antigens, Protozoan
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2002
Type:
Article
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