Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana
Biomolecules & Therapeutics
;
: 334-340, 2014.
Article
in English
| WPRIM
| ID: wpr-199227
ABSTRACT
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappaB inhibitory activity of the isolated compounds was measured in TNFalpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFalpha-stimulated NF-kappaB activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 microM. Compounds 19 and 20 also inhibited the TNFalpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 microM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saponins
/
RNA, Messenger
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Intercellular Adhesion Molecule-1
/
Inhibitory Concentration 50
/
Pulsatilla
/
Peroxisome Proliferator-Activated Receptors
/
Methanol
/
Hep G2 Cells
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Type:
Article
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