Pachymic Acid Enhances Pentobarbital-Induced Sleeping Behaviors via GABA(A)-ergic Systems in Mice
Biomolecules & Therapeutics
;
: 314-320, 2014.
Article
in English
| WPRIM
| ID: wpr-199230
ABSTRACT
This study was investigated to know whether pachymic acid (PA), one of the predominant triterpenoids in Poria cocos (Hoelen) has the sedative-hypnotic effects, and underlying mechanisms are mediated via gamma-aminobutyric acid (GABA)-ergic systems. Oral administration of PA markedly suppressed locomotion activity in mice. This compound also prolonged sleeping time, and reduced sleep latency showing synergic effects with muscimol (0.2 mg/kg) in shortening sleep onset and enhancing sleep time induced by pentobarbital, both at the hypnotic (40 mg/kg) and sub-hypnotic (28 mg/kg) doses. Additionally, PA elevated intracellular chloride levels in hypothalamic primary cultured neuronal cells of rats. Moreover, Western blotting quantitative results showed that PA increased the amount of protein level expression of GAD65/67 over a broader range of doses. PA increased alpha- and beta-subunits protein levels, but decreased gamma-subunit protein levels in GABA(A) receptors. The present experiment provides evidence for the hypnotic effects as PA enhanced pentobarbital-induced sleeping behaviors via GABA(A)-ergic mechanisms in rodents. Taken together, it is proposed that PA may be useful for the treatment of sleep disturbed subjects with insomnia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pentobarbital
/
Rodentia
/
Blotting, Western
/
Administration, Oral
/
Cocos
/
Receptors, GABA-A
/
Poria
/
Gamma-Aminobutyric Acid
/
Hypnotics and Sedatives
/
Sleep Initiation and Maintenance Disorders
Limits:
Animals
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Type:
Article
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