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Pachymic Acid Enhances Pentobarbital-Induced Sleeping Behaviors via GABA(A)-ergic Systems in Mice
Biomolecules & Therapeutics ; : 314-320, 2014.
Article in English | WPRIM | ID: wpr-199230
ABSTRACT
This study was investigated to know whether pachymic acid (PA), one of the predominant triterpenoids in Poria cocos (Hoelen) has the sedative-hypnotic effects, and underlying mechanisms are mediated via gamma-aminobutyric acid (GABA)-ergic systems. Oral administration of PA markedly suppressed locomotion activity in mice. This compound also prolonged sleeping time, and reduced sleep latency showing synergic effects with muscimol (0.2 mg/kg) in shortening sleep onset and enhancing sleep time induced by pentobarbital, both at the hypnotic (40 mg/kg) and sub-hypnotic (28 mg/kg) doses. Additionally, PA elevated intracellular chloride levels in hypothalamic primary cultured neuronal cells of rats. Moreover, Western blotting quantitative results showed that PA increased the amount of protein level expression of GAD65/67 over a broader range of doses. PA increased alpha- and beta-subunits protein levels, but decreased gamma-subunit protein levels in GABA(A) receptors. The present experiment provides evidence for the hypnotic effects as PA enhanced pentobarbital-induced sleeping behaviors via GABA(A)-ergic mechanisms in rodents. Taken together, it is proposed that PA may be useful for the treatment of sleep disturbed subjects with insomnia.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pentobarbital / Rodentia / Blotting, Western / Administration, Oral / Cocos / Receptors, GABA-A / Poria / Gamma-Aminobutyric Acid / Hypnotics and Sedatives / Sleep Initiation and Maintenance Disorders Limits: Animals Language: English Journal: Biomolecules & Therapeutics Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pentobarbital / Rodentia / Blotting, Western / Administration, Oral / Cocos / Receptors, GABA-A / Poria / Gamma-Aminobutyric Acid / Hypnotics and Sedatives / Sleep Initiation and Maintenance Disorders Limits: Animals Language: English Journal: Biomolecules & Therapeutics Year: 2014 Type: Article