Silymarin Inhibits Cytokine-Stimulated Pancreatic Beta Cells by Blocking the ERK1/2 Pathway
Biomolecules & Therapeutics
;
: 282-287, 2014.
Article
in English
| WPRIM
| ID: wpr-199235
ABSTRACT
We show that silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), inhibits cytokine mixture (CM TNF-alpha, IFN-gamma, and IL-1beta)-induced production of nitric oxide (NO) in the pancreatic beta cell line MIN6N8a. Immunostaining and Western blot analysis showed that silymarin inhibits iNOS gene expression. RT-PCR showed that silymarin inhibits iNOS gene expression in a dose-dependent manner. We also showed that silymarin inhibits extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) phosphorylation. A MEK1 inhibitor abrogated CM-induced nitrite production, similar to silymarin. Treatment of MIN6N8a cells with silymarin also inhibited CM-stimulated activation of NF-kappaB, which is important for iNOS transcription. Collectively, we demonstrate that silymarin inhibits NO production in pancreatic beta cells, and silymarin may represent a useful anti-diabetic agent.
Full text:
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Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Silymarin
/
Gene Expression
/
Blotting, Western
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Silybum marianum
/
Insulin-Secreting Cells
/
Nitric Oxide
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Type:
Article
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