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Silymarin Inhibits Cytokine-Stimulated Pancreatic Beta Cells by Blocking the ERK1/2 Pathway
Biomolecules & Therapeutics ; : 282-287, 2014.
Article in English | WPRIM | ID: wpr-199235
ABSTRACT
We show that silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), inhibits cytokine mixture (CM TNF-alpha, IFN-gamma, and IL-1beta)-induced production of nitric oxide (NO) in the pancreatic beta cell line MIN6N8a. Immunostaining and Western blot analysis showed that silymarin inhibits iNOS gene expression. RT-PCR showed that silymarin inhibits iNOS gene expression in a dose-dependent manner. We also showed that silymarin inhibits extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) phosphorylation. A MEK1 inhibitor abrogated CM-induced nitrite production, similar to silymarin. Treatment of MIN6N8a cells with silymarin also inhibited CM-stimulated activation of NF-kappaB, which is important for iNOS transcription. Collectively, we demonstrate that silymarin inhibits NO production in pancreatic beta cells, and silymarin may represent a useful anti-diabetic agent.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Silymarin / Gene Expression / Blotting, Western / NF-kappa B / Tumor Necrosis Factor-alpha / Milk Thistle / Insulin-Secreting Cells / Nitric Oxide Language: English Journal: Biomolecules & Therapeutics Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Silymarin / Gene Expression / Blotting, Western / NF-kappa B / Tumor Necrosis Factor-alpha / Milk Thistle / Insulin-Secreting Cells / Nitric Oxide Language: English Journal: Biomolecules & Therapeutics Year: 2014 Type: Article