Immunohistochemical Study of CD4 , CD8 ICAM-1 and LFA-1 in Experimental Uveitis
Journal of the Korean Ophthalmological Society
;
: 687-692, 1998.
Article
in Korean
| WPRIM
| ID: wpr-199374
ABSTRACT
We have reported an animal model of human anterior uveitis in Lewis rats with bovine melanin associated antigen[BMAA] which has been named as experimental autoimmune anterior uveitis[EAAU]. Immunopathogenesis in this model during different clinical stages was examined by the immunohistochemical detection of CD4 , CD8 , ICAM-1 and LAF-1 which play an important role in immune and inflammatory response. After the separation of eyes, these eyes were sectioned by cryostat at 6micrometer thick sections and stored at -70degreesC. For immunohistochemical study monoclonal antibodies to CD4, CD8, ICAM-1, and LAF-1 were used. An increase of ICAM-1 expression was shown on the epithelial cells of uveal tissues before clinical sign of uveitis and followed by LFA-1 expression. And there was no staining in retinal pigment epithelium and cornea. In conclusion, the immunopathogenesis of EAAU appears to be mediated by T cells and cells expressing ICAM-1, LFA-1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Uveitis
/
T-Lymphocytes
/
Uveitis, Anterior
/
Lymphocyte Function-Associated Antigen-1
/
Intercellular Adhesion Molecule-1
/
Cornea
/
Models, Animal
/
Epithelial Cells
/
Retinal Pigment Epithelium
/
Leukocytes
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
1998
Type:
Article
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