Characteristics of Intron 6 Nucleotide Sequences of cis-AB in Koreans / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 230-235, 2010.
Article
in Korean
| WPRIM
| ID: wpr-199580
ABSTRACT
BACKGROUND:
The cis-AB is a very rare phenotype in the ABO blood group system. It corresponds to a special ABO allele that encodes glycosyltransferase that is capable of synthesizing both A and B antigens. Until now, the exon 6 and 7 gene sequences of cis-AB alleles are well known. In this study, we report on the intron 6 sequence structure of the cis-AB allele.METHODS:
Standard serologic tests for the ABO blood group phenotypes were performed in four cis-AB samples. Allele-separation by cloning and subsequent sequencing was carried out.RESULTS:
The results showed that intron 6 of cis-AB is almost identical to the A101 allele except for three single nucleotide polymorphisms at nucleotide positions 163, 179 and 662, where the nucleotides of the A101 replace those of B101.CONCLUSION:
The intron 6 sequences of cis-AB in Koreans have both A101 and B101 blood group sequences.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
ABO Blood-Group System
/
Blood Grouping and Crossmatching
/
Serologic Tests
/
Base Sequence
/
Introns
/
Exons
/
Clone Cells
/
Cloning, Organism
/
Polymorphism, Single Nucleotide
Type of study:
Diagnostic study
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2010
Type:
Article
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