Flow Cytometric DNA Content Analysis in Breast Cancer Comparison study of fresh and paraffin-embedded tissues
Korean Journal of Pathology
;
: 993-999, 1998.
Article
in Korean
| WPRIM
| ID: wpr-199618
ABSTRACT
DNA content of 25 cases of breast carcinoma was analyzed by flow cytometry in both fresh and formalin-fixed, paraffin-embedded tissue. Aneuploidy in fresh tissue and paraffin-embedded tissues was 72% and 32%, respectively. There was a 52% agreement in analysis of DNA ploidy between fresh and paraffin-embedded tissues. Most of the discrepancies resulted from loss of aneuploid peaks on the histograms of paraffin-embedded tissue. Mean S-phase fraction was slightly higher in a paraffin-embedded tissue than that in the fresh tissue; 19.2 9.1% versus 16.1 8.8% and there was no significant correlation between the S-phase fractions. In statistical analysis, the histologic and nuclear grades were not correlated with ploidy or mean S-phase fraction. Therefore it is strongly recommended to use the fresh tissue in flow cytometric DNA content analysis of breast cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ploidies
/
Breast
/
Breast Neoplasms
/
DNA
/
Flow Cytometry
/
Aneuploidy
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
1998
Type:
Article
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