Isolation and Cloning of an ABC Transporter-Like Gene of Haemophilus parasuis and Its Use in a New Diagnostic PCR
Journal of Bacteriology and Virology
;
: 321-329, 2012.
Article
in English
| WPRIM
| ID: wpr-200672
ABSTRACT
The aim of this study was to identify a new gene of Haemophilus parasuis that could be used to develop a polymerase chain reaction (PCR) test for this porcine pathogen. H. parasuis genomic DNA was cloned into a set of expression vectors, and transformants expressing His-tagged polypeptides were identified by colony blotting. An ABC transporter-like gene was isolated. The cloned DNA fragment is 1,105 base pair and shows 78% similarity at the nucleotide level with an ABC transporter gene of H. ducreyi. Based on this sequence, two PCR primers were designed to amplify the entire 1,105-bp fragment in the proposed diagnostic PCR test. PCR amplification was able to detect a minimum of 1 x 10(4) CFU/ml of H. parasuis organisms. Fifteen different H. parasuis serovars were positive using the PCR test. No amplification was observed when the test was done using DNA from 16 other bacterial species commonly isolated from swine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Swine
/
DNA
/
Haemophilus
/
Polymerase Chain Reaction
/
Clone Cells
/
Cloning, Organism
/
Base Pairing
/
Haemophilus parasuis
Type of study:
Diagnostic study
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2012
Type:
Article
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