Development of Competitive ELISA for Detection of Avian Metapneumovirus Antibodies in Chicken
Journal of Bacteriology and Virology
;
: 131-143, 2010.
Article
in Korean
| WPRIM
| ID: wpr-200688
ABSTRACT
Avian metapneumovirus (aMPV) causes an acute and highly contagious upper respiratory tract infection in turkeys and chickens. In this study, a competitive ELISA (C-ELISA) was developed for the detection of antibodies to aMPV in chicken sera and/or their egg yolks. This assay is based on the competitive binding of monoclonal antibody with serum antibodies to recombinant aMPV N protein expressed by a recombinant baculovirus. The C-ELISA showed specificity and sensitivity of 100% and 98.0%, respectively, when compared to the virus neutralization test. In specific pathogen-free chickens experimentally infected with aMPV SC1509 strain, the C-ELISA started to detect antibodies to aMPV as early as 5 days post infection from birds infected with aMPV, while a commercial ELISA kit detected first 10 days post infection. The C-ELISA was similar or superior to a commercial ELISA kit when serum and egg yolk samples collected from chickens on six outbreak farms were tested for diagnosis. The C-ELISA developed in the present work provides a short turnaround time and can be a useful diagnostic and screening tool for aMPV infection in the field.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Respiratory Tract Infections
/
Sprains and Strains
/
Turkeys
/
Viruses
/
Binding, Competitive
/
Birds
/
Enzyme-Linked Immunosorbent Assay
/
Neutralization Tests
/
Chickens
/
Mass Screening
Type of study:
Diagnostic study
/
Screening study
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2010
Type:
Article
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