Evaluation of cytolytic activity and phenotypic changes of circulating blood immune cells in patients with colorectal cancer by a simple preparation of peripheral blood mononuclear cells / 대한외과학회지
Journal of the Korean Surgical Society
;
: 230-235, 2013.
Article
in English
| WPRIM
| ID: wpr-200749
ABSTRACT
PURPOSE:
This study aimed to assess the cytolytic activity and the phenotype of circulating blood immune cells in cancer patients by using a simple preparation of peripheral blood mononuclear cells (PBMCs).METHODS:
Peripheral blood was obtained from 94 diagnosed colorectal cancer (CRC) patients and 112 healthy donors. PBMCs were cocultured with K562 cells for 2 hours and lactate dehydrogenase released from the dead K562 cells was measured by using a spectrophotometer. Meanwhile, PBMCs were stained with fluorescence conjugated monoclonal antibodies (mAbs) and analyzed by flow cytometry.RESULTS:
The cytolytic activity of PBMCs were significantly different between CRC patient and healthy groups (8.82% +/- 3.84% vs. 17.51% +/- 8.57%; P < 0.001). However, no significant difference in the cytolytic activity was observed after surgery in the CRC patient group (before surgery, 8.82% +/- 3.84% vs. after surgery, 9.95% +/- 4.94%; P = 0.326). In addition, the percentage of peripheral blood natural killer cells was significantly higher in the preoperative patient group than in the healthy group (19.97% +/- 11.51% vs. 15.60% +/- 5.77%, P = 0.041). In contrast, the percentage of peripheral blood lymphocytes was lower in the preoperative patient group than in the healthy group (28.41% +/- 8.31% vs. 36.4% +/- 8.6%, P < 0.001).CONCLUSION:
These results demonstrate that circulating blood immune cells of CRC patients are functionally impaired and undergo an immunophenotypic perturbation, and show that a simple preparation of PBMCs can be useful to evaluate cellular immunity in cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Tissue Donors
/
Blood Cells
/
Killer Cells, Natural
/
Lymphocytes
/
Colorectal Neoplasms
/
K562 Cells
/
Cytotoxicity, Immunologic
/
Fluorescence
/
Immunity, Cellular
Limits:
Humans
Language:
English
Journal:
Journal of the Korean Surgical Society
Year:
2013
Type:
Article
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