The expression and cellular localization of phospholipase D isozymes in the developing mouse testis
Journal of Veterinary Science
; : 209-212, 2007.
Article
in En
| WPRIM
| ID: wpr-200811
Responsible library:
WPRO
ABSTRACT
To examine the involvement of phospholipase D (PLD)isozymes in postnatal testis development, the expression ofPLD1 and PLD2 was examined in the mouse testis atpostnatal weeks 1, 2, 4, and 8 using Western blot analysisand immunohistochemistry. The expression of both PLD1and PLD2 increased gradually with development frompostnatal week 1 to 8. Immunohistochemically, PLDimmunoreactivity was detected in some germ cells in thetestis and interstitial Leydig cells at postnatal week 1.PLD was mainly detected in the spermatocytes andresidual bodies of spermatids in the testis after 8 weeksafter birth. The intense immunostaining of PLD in Leydigcells remained unchanged by postnatal week 8. Thesefindings suggest that PLD isozymes are involved in thespermatogenesis of the mouse testis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phospholipase D
/
Spermatogenesis
/
Testis
/
Immunohistochemistry
/
Blotting, Western
/
Isoenzymes
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Mice, Inbred BALB C
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2007
Type:
Article