Auto-antibody Showing Anti-Fy(b) Specificity as Proven by the Dilution Method in the Presence of Warm Autoantibodies: A Case Report / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 74-79, 2010.
Article
in Korean
| WPRIM
| ID: wpr-200867
ABSTRACT
Several approaches have been introduced to detect allo-antibodies in the presence of warm auto-antibodies, and these methods include warm autoadsorption, cysteine-activated papain and dithiothreitol (ZZAP), and polyethylene glycol (PEG) and dilution of the patient's serum. Among them, the dilution technique is a simple and rapid method. During pretransfusion testing of a 33 year-old systemic lupus erythematosus (SLE) patient with warm auto-antibodies, antibody identification was done by the dilution technique with using serum diluted 1-in-8. The patient demonstrated an anti-Fy(b) pattern of reactivity in his sera. Contrary to our expectations, the phenotype of the erythrocytes was Fy(a+/b+) and the genotype, as assessed by performing PCR-restriction fragment length polymorphism (RFLP), was FY*A/FY*B. These results suggest that the antibody is an autoantibody showing anti-Fy(b) specificities. An antibody identification test using undiluted serum showed the same result when 40 days had passed. We report here on a case with auto-anti-Fy(b) proven by the dilution method in the presence of warm autoantibodies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Polyethylene Glycols
/
Autoantibodies
/
Papain
/
Indicator Dilution Techniques
/
Sensitivity and Specificity
/
Dithiothreitol
/
Erythrocytes
/
Genotype
/
Lupus Erythematosus, Systemic
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2010
Type:
Article
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