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Changes of Nitric Oxide and Cell Proliferation after Bacillus Calmette-Guerin and Nitric Oxide Synthase Inhibitor Treatment on Murine Bladder Tumor-2 Cell Line / 대한비뇨기과학회지
Korean Journal of Urology ; : 155-159, 2003.
Article in Korean | WPRIM | ID: wpr-202042
ABSTRACT

PURPOSE:

We hypothesize that the cytolytic activity of Bacillus Calmette-Guerin (BCG) may act through nitric oxide (NO) production, and that cell death is correlated with apoptosis, changes of NO and cell proliferation following BCG and/or nitric oxide synthase (NOS) inhibition treatment of a murine bladder tumor-2 (MBT-2) cell line. If these cell lines show cell death due to apoptosis was also determined. MATERIALS AND

METHODS:

NO production and proliferation activity of the MBT-2 cell line were measured after stimulation, with BCG and/or L-NAME, using an enzyme- linked immunosorbent assay (ELISA). After incubation of the MBT-2 cells with BCG and/or L-NAME, the cell cycle was analysis was performed by immunocytometry.

RESULTS:

The production of NO in the MBT-2 cells was significantly increased by the BCG. The BCG had direct dose-dependent cytotoxic effects on the MBT-2 cell line. After the L-NAME treatment, both the NO production and cytotoxicity were decreased (p<0.05). When the MBT-2 cells were cultured with BCG, the apoptotic cell ratio increased compared to that of the MBT-2 cells treated with L-NAME (p<0.05).

CONCLUSIONS:

The up-regulation of the production of NO following BCG treatment of the MBT-2 cell line may be due, in part, to the cytolytic action of the BCG. The cell death, when BCG was instilled, correlated with apoptosis.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacillus / Urinary Bladder / Cell Cycle / Up-Regulation / Cell Line / Cell Death / Apoptosis / Nitric Oxide Synthase / NG-Nitroarginine Methyl Ester / Cell Proliferation Language: Korean Journal: Korean Journal of Urology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacillus / Urinary Bladder / Cell Cycle / Up-Regulation / Cell Line / Cell Death / Apoptosis / Nitric Oxide Synthase / NG-Nitroarginine Methyl Ester / Cell Proliferation Language: Korean Journal: Korean Journal of Urology Year: 2003 Type: Article