Changes of Nitric Oxide and Cell Proliferation after Bacillus Calmette-Guerin and Nitric Oxide Synthase Inhibitor Treatment on Murine Bladder Tumor-2 Cell Line / 대한비뇨기과학회지
Korean Journal of Urology
;
: 155-159, 2003.
Article
in Korean
| WPRIM
| ID: wpr-202042
ABSTRACT
PURPOSE:
We hypothesize that the cytolytic activity of Bacillus Calmette-Guerin (BCG) may act through nitric oxide (NO) production, and that cell death is correlated with apoptosis, changes of NO and cell proliferation following BCG and/or nitric oxide synthase (NOS) inhibition treatment of a murine bladder tumor-2 (MBT-2) cell line. If these cell lines show cell death due to apoptosis was also determined. MATERIALS ANDMETHODS:
NO production and proliferation activity of the MBT-2 cell line were measured after stimulation, with BCG and/or L-NAME, using an enzyme- linked immunosorbent assay (ELISA). After incubation of the MBT-2 cells with BCG and/or L-NAME, the cell cycle was analysis was performed by immunocytometry.RESULTS:
The production of NO in the MBT-2 cells was significantly increased by the BCG. The BCG had direct dose-dependent cytotoxic effects on the MBT-2 cell line. After the L-NAME treatment, both the NO production and cytotoxicity were decreased (p<0.05). When the MBT-2 cells were cultured with BCG, the apoptotic cell ratio increased compared to that of the MBT-2 cells treated with L-NAME (p<0.05).CONCLUSIONS:
The up-regulation of the production of NO following BCG treatment of the MBT-2 cell line may be due, in part, to the cytolytic action of the BCG. The cell death, when BCG was instilled, correlated with apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacillus
/
Urinary Bladder
/
Cell Cycle
/
Up-Regulation
/
Cell Line
/
Cell Death
/
Apoptosis
/
Nitric Oxide Synthase
/
NG-Nitroarginine Methyl Ester
/
Cell Proliferation
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2003
Type:
Article
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