Smad6 Gene and Suppression of Radiation-Induced Apoptosis by Genistein in K562 cells / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
;
: 245-251, 2001.
Article
in Korean
| WPRIM
| ID: wpr-202273
ABSTRACT
PURPOSE:
The genes involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. MATERIALS ANDMETHODS:
K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. Forx-ray irradiation and drug treatment, cultures were prepared at 2x105 cells/mL. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA). Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at 37degreesC for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined.RESULTS:
Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis.CONCLUSION:
We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Accelerators
/
RNA, Messenger
/
Leukemia
/
Cell Line
/
Dimethyl Sulfoxide
/
Sequence Analysis, DNA
/
Apoptosis
/
DNA, Complementary
/
Genistein
/
K562 Cells
Language:
Korean
Journal:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Year:
2001
Type:
Article
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