Valproic Acid Regulates alpha-Synuclein Expression through JNK Pathway in Rat Primary Astrocytes
Biomolecules & Therapeutics
;
: 222-228, 2013.
Article
in English
| WPRIM
| ID: wpr-202360
ABSTRACT
Although the role of alpha-synuclein aggregation on Parkinson's disease is relatively well known, the physiological role and the regulatory mechanism governing the expression of alpha-synuclein are unclear yet. We recently reported that alpha-synuclein is expressed and secreted from cultured astrocytes. In this study, we investigated the effect of valproic acid (VPA), which has been suggested to provide neuroprotection by increasing alpha-synuclein in neuron, on alpha-synuclein expression in rat primary astrocytes. VPA concentration-dependently increased the protein expression level of alpha-synuclein in cultured rat primary astrocytes with concomitant increase in mRNA expression level. Likewise, the level of secreted alpha-synuclein was also increased by VPA. VPA increased the phosphorylation of Erk1/2 and JNK and pretreatment of a JNK inhibitor SP600125 prevented the VPA-induced increase in alpha-synuclein. Whether the increased alpha-synuclein in astrocytes is involved in the reported neuroprotective effects of VPA awaits further investigation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
Phosphorylation
/
Acetylation
/
RNA, Messenger
/
Astrocytes
/
Valproic Acid
/
Neuroprotective Agents
/
MAP Kinase Signaling System
/
Alpha-Synuclein
/
Neurons
Limits:
Animals
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS