Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD)
Journal of Veterinary Science
;
: 429-432, 2012.
Article
in English
| WPRIM
| ID: wpr-202775
ABSTRACT
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stress, Psychological
/
Brain
/
Blotting, Western
/
Mice, Knockout
/
Wasting Disease, Chronic
/
Antibodies, Monoclonal
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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