In silico analysis for identifying potential vaccine candidates against Staphylococcus aureus
Clinical and Experimental Vaccine Research
;
: 99-106, 2015.
Article
in English
| WPRIM
| ID: wpr-203145
ABSTRACT
PURPOSE:
Staphylococcus aureus is one of the most important causes of nosocomial and community-acquired infections. The increasing incidence of multiple antibiotic-resistant S. aureus strains and the emergence of vancomycin resistant S. aureus strains have placed renewed interest on alternative means of prevention and control of infection. S. aureus produces a variety of virulence factors, so a multi-subunit vaccine will be more successful for preventing S. aureus infections than a mono-subunit vaccine. MATERIALS ANDMETHODS:
We selected three important virulence factors of S. aureus, clumping factor A (ClfA), iron-regulated surface determinant (IsdB), and gamma hemolysin (Hlg) that are potential candidates for vaccine development. We designed synthetic genes encoding the clfA, isdB, and hlg and used bioinformatics tools to predict structure of the synthetic construct and its stabilities. VaxiJen analysis of the protein showed a high antigenicity. Linear and conformational B-cell epitopes were identified.RESULTS:
The proteins encoded by these genes were useful as vaccine candidates against S. aureus infections.CONCLUSION:
In silico tools are highly suited to study, design, and evaluate vaccine strategies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Staphylococcus aureus
/
Computer Simulation
/
Vaccines
/
Vancomycin
/
Incidence
/
Community-Acquired Infections
/
Epitopes, B-Lymphocyte
/
Computational Biology
/
Virulence Factors
/
Genes, Synthetic
Type of study:
Incidence study
/
Prognostic study
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2015
Type:
Article
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