Role of Rho-Kinase Activity in Angiotensin II-Induced Contraction of Corpus Cavernosum Smooth Muscle in the Rabbit / 대한남성과학회지
Korean Journal of Andrology
;
: 32-37, 2003.
Article
in Korean
| WPRIM
| ID: wpr-203310
ABSTRACT
PURPOSE:
RhoA/Rho-kinase regulates vascular tone via a calcium sensitization mechanism. Stimulation of the AT1 receptor by angiotensin (ANG) II activates the Rho A/Rho-kinase signaling pathway. However, its role in corpus cavernosum smooth muscle (PCSM) has not been known. MATERIALS ANDMETHODS:
Isometric tension measurements were performed in rabbit PCSM using a selective Rho-kinase inhibitor, (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide (Y-27632), and a selective myosin light chain kinase (MLCK) inhibitor, 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML7).RESULTS:
Y-27632 significantly attenuated contractions induced by ANG II in a dose-dependent fashion. However, ML7 did not affect the contractile response to ANG II except at high concentration. Y-27632 inhibited contraction in response to phenylephrine (PhE), but ML7 did not. A nitric oxide synthase inhibitor, NG-nitro-L-arginine-methyl ester, did not affect Y-27632-induced relaxation of the strip contracted with PhE.CONCLUSIONS:
A G-protein-coupled increase in myofilament Ca2+ sensitivity, mediated through the RhoA/Rho-kinase signaling pathway, is involved in the regulation of the PCSM tone induced by ANG II. The RhoA/Rho-kinase pathway acts in AGN II-induced contraction independent of the NO pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenylephrine
/
Relaxation
/
Myosin-Light-Chain Kinase
/
Angiotensin II
/
Angiotensins
/
Calcium
/
Nitric Oxide Synthase
/
Rho-Associated Kinases
/
Muscle, Smooth
/
Myofibrils
Language:
Korean
Journal:
Korean Journal of Andrology
Year:
2003
Type:
Article
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