Effect of Dietary Calcium on Spinal Bone Fusion in an Ovariectomized Rat Model
Journal of Korean Neurosurgical Society
;
: 281-287, 2012.
Article
in English
| WPRIM
| ID: wpr-203499
ABSTRACT
OBJECTIVE:
To evaluate the effect of calcium supplementation on spinal bone fusion in ovariectomized (OVX) rats.METHODS:
Sixteen female Sprague Dawley rats underwent bilateral ovariectomy at 12 weeks of age to induce osteoporosis and were randomly assigned to two groups control group (n=8) and calcium-supplemented group (OVX-Ca, n=8). Autologous spinal bone fusion surgery was performed on both groups 8 weeks later. After fusion surgery, the OVX-Ca group was supplemented with calcium in drinking water for 8 weeks. Blood was obtained 4 and 8 weeks after fusion surgery. Eight weeks after fusion surgery, the rats were euthanized and the L4-5 spine removed. Bone fusion status and fusion volume were evaluated by manual palpation and three-dimensional computed tomography.RESULTS:
The mean fusion volume in the L4-5 spine was significantly greater in the OVX-Ca group (71.80+/-8.06 mm3) than in controls (35.34+/-8.24 mm3) (p<0.01). The level of osteocalcin, a bone formation marker, was higher in OVX-Ca rats than in controls 4 weeks (610.08+/-10.41 vs. 551.61+/-12.34 ng/mL) and 8 weeks (552.05+/-19.67 vs. 502.98+/-22.76 ng/mL) after fusion surgery (p<0.05). The level of C-terminal telopeptide fragment of type I collagen, a bone resorption marker, was significantly lower in OVX-Ca rats than in controls 4 weeks (77.07+/-12.57 vs. 101.75+/-7.20 ng/mL) and 8 weeks (69.58+/-2.45 vs. 77.15+/-4.10 ng/mL) after fusion surgery (p<0.05). A mechanical strength test showed that the L4-5 vertebrae in the OVX-Ca group withstood a 50% higher maximal load compared with the controls (p<0.01).CONCLUSION:
Dietary calcium given to OVX rats after lumbar fusion surgery improved fusion volume and mechanical strength in an ovariectomized rat model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Osteoporosis
/
Palpation
/
Spine
/
Drinking Water
/
Bone Resorption
/
Calcium, Dietary
/
Ovariectomy
/
Osteocalcin
/
Calcium
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2012
Type:
Article
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