Macrophage colony-stimulating factor promotes the survival of osteoclast precursors by up-regulating Bcl-XL
Experimental & Molecular Medicine
;
: 340-346, 2002.
Article
in English
| WPRIM
| ID: wpr-203703
ABSTRACT
Macrophage colony-stimulating factor (M-CSF) is known as one of the factors essential for osteoclast development. In the present study, we examined effects of M-CSF on the apoptotic pathway of osteoclast precursors and their underlying molecular mechanisms. Osteoclast precursors underwent apoptosis in the absence of M-CSF, even in the presence of receptor activator of NF-kB ligand (RANKL). Active caspase-3 and -9 were detected in the osteoclast precursors and treatments of precursors with their specific inhibitors (Z- DEVD-FMK and Z-LEHD-FMK) decreased the apoptosis. M-CSF decreased apoptosis in a dose-dependent manner with decreasing in active caspases-3 and -9 levels and up-regulating Bcl-XL. Those effects of M-CSF on inhibiting apoptosis of osteoclasts precursor by regulating anti-apoptotic signals was more effective when combined with RANKL. These results demonstrate that M-CSF acts as a survival factor for the osteoclast precursors. Furthermore, it is believed that the apoptosis of osteoclast precursors may be involved in the activation of caspase-9 and that M-CSF may promote their survival through Bcl-XL-induced inhibition of caspase-9 activation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Osteoclasts
/
Stem Cells
/
Membrane Glycoproteins
/
Carrier Proteins
/
Cysteine Proteinase Inhibitors
/
Up-Regulation
/
Cell Survival
/
Cells, Cultured
/
Macrophage Colony-Stimulating Factor
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2002
Type:
Article
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