Immunohistochemical Study for the Syndecan-2 in Cutaneous Melanoma and Pigmented Nevus / 대한피부과학회지
Korean Journal of Dermatology
;
: 776-781, 2013.
Article
in Korean
| WPRIM
| ID: wpr-204077
ABSTRACT
BACKGROUND:
Melanoma is a malignant neoplasm originating from melanocytes. It has been recently suggested that syndecan-2 may contribute to the aggressive phenotype and metastatic potential of melanoma in cell line studies. However, there is no quantitative analysis of syndecan-2 expression using human melanoma tissue.OBJECTIVE:
This study aimed to examine the specific expression of syndecan-2 in human melanoma tissue.METHODS:
A total of 35 sections of formalin-fixed, paraffin-embedded tissues were investigated for syndecan-2 expression using immunohistochemical staining. Also, a total of 6 tissues and two kinds of cell lines were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) and western blot.RESULTS:
Immunohistochemical staining of 23 cases of melanoma tissue was done, and in 5 cases (21.7%), strong expression of syndecan-2 was seen. Also, syndecan-2 was detected in human melanoma tissue and MNT-1 melanoma cells by RT-PCR and western blot analysis.CONCLUSION:
We suggest that syndecan-2 expression is increased in melanoma compared to nevus. The results of this study may help to explain the clinical features of melanoma and syndecan-2.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Cell Line
/
Blotting, Western
/
Polymerase Chain Reaction
/
Syndecan-2
/
Melanocytes
/
Melanoma
/
Nevus
/
Nevus, Pigmented
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
2013
Type:
Article
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