Regulation of Tumor Necrosis Factor-alpha-induced Airway Mucin Production and Gene Expression by Carbenoxolone, Prunetin, and Silibinin / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 348-353, 2010.
Article
in English
| WPRIM
| ID: wpr-204137
ABSTRACT
BACKGROUND:
In this study, we tried to investigate whether carbenoxolone, prunetin, and silibinin affect tumor necrosis factor (TNF)-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells.METHODS:
Confluent NCI-H292 cells were pretreated with each agent (carbenoxolone, prunetin, and silibinin) for 30 min and then stimulated with TNF-alpha for 24 hours. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay, respectively.RESULTS:
Carbenoxolone, prunetin and silibinin inhibited the production of MUC5AC mucin protein induced by TNF-alpha; the 3 compounds also inhibited the expression of MUC5AC mucin gene induced by TNF-alpha.CONCLUSION:
This result suggests that carbenoxolone, prunetin and silibinin can inhibit mucin gene expression and production of mucin protein induced by TNF-alpha, by directly acting on airway epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Silymarin
/
Enzyme-Linked Immunosorbent Assay
/
Carbenoxolone
/
Gene Expression
/
Tumor Necrosis Factor-alpha
/
Epithelial Cells
/
Mucin 5AC
/
Isoflavones
/
Mucins
/
Necrosis
Limits:
Humans
Language:
English
Journal:
Tuberculosis and Respiratory Diseases
Year:
2010
Type:
Article
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