Hyperglycemia decreases preoxiredoxin-2 expression in a middle cerebral artery occlusion model / 한국실험동물학회지
Laboratory Animal Research
;
: 98-104, 2017.
Article
in English
| WPRIM
| ID: wpr-204556
ABSTRACT
Diabetes is a major risk factor for stroke and is also associated with worsened outcomes following a stroke. Peroxiredoxin-2 exerts potent neuroprotective effects against oxidative stress. In the present study, we identified altered peroxiredoxin-2 expression in an ischemic stroke model under hyperglycemic conditions. Adult male rats were administrated streptozotocin (40 mg/kg) via intraperitoneal injection to induce diabetes. Middle cerebral artery occlusion (MCAO) was induced surgically 4 weeks after streptozotocin treatment and cerebral cortex tissues were isolated 24 hours after MCAO. Peroxiredoxin-2 expression was evaluated in the cerebral cortex of MCAO-operated animals using a proteomics approach, and was found to be decreased. In addition, the reduction in peroxiredoxin-2 levels was more severe in cerebral ischemia with diabetes compared to animals without diabetes. Reverse-transcriptase PCR and Western blot analyses confirmed the significantly reduced peroxiredoxin-2 expression in MCAO-operated animals under hyperglycemic conditions. It is an accepted fact that peroxiredoxin-2 has antioxidative activity against ischemic injury. Thus, the findings of this study suggest that a more severe reduction in peroxiredoxin-2 under hyperglycemic conditions leads to worsened brain damage during cerebral ischemia with diabetes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Cerebral Cortex
/
Brain Ischemia
/
Blotting, Western
/
Polymerase Chain Reaction
/
Risk Factors
/
Streptozocin
/
Oxidative Stress
/
Neuroprotective Agents
/
Middle Cerebral Artery
Type of study:
Etiology study
/
Prognostic study
/
Risk factors
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Laboratory Animal Research
Year:
2017
Type:
Article
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