Neuroprotective Effect of Resveratrol on Acute Brain Ischemia Reperfusion Injury by Measuring Annexin V, p53, Bcl-2 Levels in Rats
Journal of Korean Neurosurgical Society
;
: 508-512, 2015.
Article
in English
| WPRIM
| ID: wpr-204845
ABSTRACT
BACKGROUND:
Cerebral ischemia is as a result of insufficient cerebral blood flow for cerebral metabolic functions. Resveratrol is a natural phytoalexin that can be extracted from grape's skin and had potent role in treating the cerebral ischemia. Apoptosis, a genetically programmed cellular event which occurs after ischemia and leads to biochemical and morphological changes in cells. There are some useful markers for apoptosis like Bcl-2, bax, and p53. The last reports, researchers verify the apoptosis with early markers like Annexin V.METHODS:
We preferred in this experimental study a model of global cerebral infarction which was induced by bilateral common carotid artery occlusion method. Rats were randomly divided into 4 groups sham, ischemia-reperfusion (I/R), I/R plus 20 mg/kg resveratrol and I/R plus 40 mg/kg resveratrol. Statistical analysis was performed using Sigmastat 3.5 ve IBM SPSS Statistics 20. We considered a result significant when p<0.001.RESULTS:
After administration of resveratrol, Bcl-2 and Annexin levels were significantly increased (p<0.001). Depending on the dose of resveratrol, Bcl2 levels increased, p53 levels decreased but Annexin V did not effected. P53 levels were significantly increased in ishemia group, so apoptosis is higher compared to other groups.CONCLUSION:
In the acute period, Annexin V levels misleading us because the apoptotic cell counts could not reach a certain level. Therefore we should support our results with bcl-2 and p53.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Brain
/
Reperfusion
/
Reperfusion Injury
/
Cell Count
/
Cerebral Infarction
/
Brain Ischemia
/
Carotid Artery, Common
/
Apoptosis
/
Annexin A5
Limits:
Animals
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2015
Type:
Article
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