Mixture Patterned Short Tandem Repeat Profile in a Perimortem Transfused Patient / 대한법의학회지
Korean Journal of Legal Medicine
;
: 27-31, 2016.
Article
in English
| WPRIM
| ID: wpr-204992
ABSTRACT
Recently, it has been reported that transfused patients can generate admixture-like genetic profiles. As genetic material of the donor can survive for a reasonable time after transfusion, the recipient's genomic DNA is likely to have a mixture pattern. An autopsy case of a man transfused perimortem generated a mixture patterned short tandem repeat profile. Notably, the patient was transfused mostly with nuclear-deficient cells, limiting the donor genetic material available for the recipient. As a result, mixture-like patterns were observed consistently, regardless of change in input DNA content; the sample DNA content, which was serially diluted, ranged from 1 ng to 0.0625 ng. The distributions of foreign peaks appeared to be irreproducible, showing stochastic behaviors throughout the genotyped results. This study suggests that a cautious approach is required when genotyping of a patient who has undergone recent transfusion. One must consider the possibility of obtaining a mixture patterned profile in such patients, and therefore, choose parenchymal organs or tissues for reliable results.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autopsy
/
Tissue Donors
/
Blood Transfusion
/
DNA
/
DNA Fingerprinting
/
Microsatellite Repeats
Limits:
Humans
Language:
English
Journal:
Korean Journal of Legal Medicine
Year:
2016
Type:
Article
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