The mechanism of mesna in protection from cisplatin-induced ovarian damage in female rats / 부인종양
Journal of Gynecologic Oncology
;
: 177-185, 2013.
Article
in English
| WPRIM
| ID: wpr-205184
ABSTRACT
OBJECTIVE:
Cisplatin is a widely used chemotherapeutic agent in the treatment of cancers in clinic; but it often induces adverse effects on ovarian functions such as reduced fertility and premature menopause. Mesna could attenuate the cisplatin-induced ovarian damages; however, the underlying mechanism is still unknown. This study aimed to figure out the underlying mechanism of the protection of mesna for ovaries against cisplatin therapy in cancers.METHODS:
We performed female adult Sprague-Dawley rats into normal saline control (NS), low-dose cisplatin (CL), high-dose cisplatin (CH), CL plus mesna (CL+M), and CH plus mesna (CH+M) groups and detected anti-Mullerian hormone (AMH)-positive follicle, oxidative stress status and anti-oxidative capability in ovaries.RESULTS:
AMH-positive follicles were significantly decreased after cisplatin administration, which was significantly reversed when mesna was co-administered with cisplatin. The end product of lipid peroxidation, malondialdehyde (MDA), was significantly increased, but the anti-oxidative enzymatic activity of superoxide dismutase (SOD) and glutathione (GSH) were significantly decreased in cisplatin groups when compared with NS group. In contrast, after co-administration of cisplatin with mesna, MDA was significantly decreased whereas the activity of SOD and the concentration of GSH were increased. Moreover, mesna did not decrease the anti-tumor property of cisplatin in HePG2 cell lines.CONCLUSION:
Cisplatin damages the granulosa cells by oxidative stress to deplete the ovarian reserve and mesna could protect ovarian reserve through anti-oxidation. These results might highlight the mechanism of the protection of mesna for ovarian reserve and open an avenue for the application of mesna as a protective additive in cisplatin chemotherapy in clinical practise.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovary
/
Superoxide Dismutase
/
Menopause, Premature
/
Lipid Peroxidation
/
Cisplatin
/
Mesna
/
Rats, Sprague-Dawley
/
Oxidative Stress
/
Anti-Mullerian Hormone
/
Hep G2 Cells
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Journal of Gynecologic Oncology
Year:
2013
Type:
Article
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