Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis
The Korean Journal of Parasitology
;
: 121-123, 2003.
Article
in English
| WPRIM
| ID: wpr-206118
ABSTRACT
This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoblotting
/
Helminth Proteins
/
Electrophoresis, Polyacrylamide Gel
/
Fasciola hepatica
/
Fascioliasis
/
Antigens, Helminth
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2003
Type:
Article
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