Mechanism of Toxic Shock Syndrome Toxin-1 as a Superantigen / 감염
Korean Journal of Infectious Diseases
;
: 134-142, 1998.
Article
in Korean
| WPRIM
| ID: wpr-207065
ABSTRACT
BACKGROUND:
Staphylococcus aureus TSST-1 is a microbial superantigen which requires interaction between MHC class II molecules and T-cell receptors. To characterize the mechanism of TSST-1 as a superantigen, we investigated the influence of TSST-1 on the binding of T-cell receptor and MHC II.METHODS:
For control, A20 cells and lymphocytes from healthy volunteers were measured by the IL-2 bioassay with or withtout TSST-1, which was then purified and confirmed using immunoblot. mRNA for TNF from PBMC was prepared and RT-PCR for mRNA expression was performed.RESULTS:
When stimulated by TSST-1(100pg/ mL), lymphocytes produced 135microgram/ml of IL-2 compared to the control A20 cells which produced 5microgram/ ml of 1L-2. TSST-1 was confirmed to be the strong inducer for mRNA expression by RT-PCR.CONCLUSION:
The binding to T-cell receptor was enhanced by TSST-1 as confirmed by the bioassay of IL-2. This data may contribute to the understanding of mechanism of cell-toxin interaction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Shock, Septic
/
Staphylococcus aureus
/
Biological Assay
/
RNA, Messenger
/
Lymphocytes
/
Receptors, Antigen, T-Cell
/
Interleukin-2
/
Healthy Volunteers
Language:
Korean
Journal:
Korean Journal of Infectious Diseases
Year:
1998
Type:
Article
Similar
MEDLINE
...
LILACS
LIS