Analysis of extended-spectrum beta-lactamase TEM produced by Klebsiella Pneumoniae / 감염
Korean Journal of Infectious Diseases
;
: 126-133, 1998.
Article
in Korean
| WPRIM
| ID: wpr-207066
ABSTRACT
BACKGROUND:
The prevalence of extended-spectrum beta-lactamase(ESBL)-producing organisms has been increasing in Korea. We performed a study to characterize various TEM derivatives with clinical isolates of Klebsiella pneumoniae collected from 3 hospitals in Korea.METHODS:
Fifty-seven isolates of ESBL-producing K. pneumoniae collected from 3 hospitals were screened by TEM-specific PCR for the carriage of TEM genes. Thirteen strains were found to have TEM-related genes. Eleven blaTEM genes were amplifed and sequenced. The transfer of resistance was tested by conjugation and isoelectric points of beta-lactamases were determined. MICs were measured to obtain a resistance pattern for each indivudual wild- type and transconjugant strain. The hydrolysis rate of TEM-52 was measured spectrophotometrically.RESULTS:
Ten strains carried plasmid-mediated CTEM-52 gene, which sequence showed the substitution of 3 amino acids compared to that of TEM-1 104 glutamic acid --> lysine (GAG --> AAG), 182 methionine --> threonine (ATG --> ACG), and 238 glycine --> serine (GGT --> AGT). MIC showed that TEM-52 mediated a resistance to ceftazidime and aztreonam at a lower level than to cefotaxime. TEM-52 enzyme hydrolyzed cefotaxime efficiently (Vmax, 340) and showed fairly weak activity for ceftazidime (Vmax, 15.1), but very weak activity for aztreonam. The gene of TEM-52 beta-lactamases was mediated by approximately 77-kb plasmids.CONCLUSION:
From these results, we conclude that the TEM-52 beta-lactamase is a common TEM- type ESBL in K. pneumoniae in Korea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penicillinase
/
Plasmids
/
Pneumonia
/
Serine
/
Threonine
/
Beta-Lactamases
/
Aztreonam
/
Cefotaxime
/
Ceftazidime
/
Polymerase Chain Reaction
Type of study:
Prevalence study
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Infectious Diseases
Year:
1998
Type:
Article
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