Profiling of antimicrobial resistance and plasmid replicon types in beta-lactamase producing Escherichia coli isolated from Korean beef cattle
Journal of Veterinary Science
;
: 483-489, 2015.
Article
in English
| WPRIM
| ID: wpr-207356
ABSTRACT
In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum beta-lactamase (ESBL) and/or AmpC beta-lactamase. In the disc diffusion test with ampicillin, amoxicillin, cephalothin, ceftiofur, cefotaxime, ceftazidime, and cefoxitin, 38.5% of the isolates showed resistance to all of ampicillin, amoxicillin, and cephalothin. The double disc synergy method revealed that none of the isolates produced ESBL or AmpC beta-lactamases. DNA sequencing showed that all isolates encoded genes for TEM-1-type beta-lactamase. Moreover, 78.2% of the isolates transferred the TEM-1-type beta-lactamase gene via conjugation. In plasmid replicon typing of all donors, IncFIB and IncFIA were identified in 71.4% and 41.0% of plasmids, respectively. In transconjugants, IncFIB and IncFIA were the most frequent types detected (61.5% and 41.0%, respectively). Overall, the present study indicates that selection pressures of antimicrobials on beta-lactamases in beef cattle may be low relative to other livestock animals in Korea. Moreover, to reduce selection pressure and dissemination of beta-lactamase, the long-term surveillance of antimicrobial use in domestic beef cattle should be established.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Replicon
/
Tissue Donors
/
Beta-Lactamases
/
Cefotaxime
/
Cefoxitin
/
Ceftazidime
/
Cephalothin
/
Sequence Analysis, DNA
/
Diffusion
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Veterinary Science
Year:
2015
Type:
Article
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