Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea
The Korean Journal of Parasitology
;
: 631-636, 2016.
Article
in English
| WPRIM
| ID: wpr-207490
ABSTRACT
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively. The multiplex-touchdown PCR protocol using a primer mixture simultaneously detected protozoa in human stools, and the amplified gene was detected in >1×10³ oocysts for C. parvum, >1×10⁴ cysts for G. lamblia, and >1 copy of the 18S rRNA gene for C. cayetanensis. Taken together, our protocol convincingly demonstrated the ability to simultaneously detect C. parvum, G. lamblia, and C. cayetanenesis in stool samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasites
/
RNA, Ribosomal, 18S
/
Polymerase Chain Reaction
/
Giardia lamblia
/
Cryptosporidium parvum
/
Genes, rRNA
/
Cryptosporidium
/
Cyclospora
/
Oocysts
/
Diarrhea
Type of study:
Etiology study
/
Practice guideline
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2016
Type:
Article
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