The Neuroprotective Effect of Intravitreally Injected CNTF on Rat Retinal Ganglion Cell in Optic Nerve Crush Injury Model
Journal of the Korean Ophthalmological Society
;
: 491-495, 2003.
Article
in Korean
| WPRIM
| ID: wpr-207751
ABSTRACT
PURPOSE:
To make an optic nerve crush injury model and to investigate the neuroprotective effect of intravitreally injected ciliary neurotrophic factor (CNTF) on rat retinal ganglion cells (RGCs) in the model.METHODS:
The optic nerves of 12 Sprague-Dawley rats were crushed at 3 mm posterior to the eyeball for 1 minute using aneurysm clip (110 g). Two micrograms of CNTF in 2 micro liter of vehicle was injected intravitreally in one group (n=6) and 2 micro liter of PBS was injected in the control group (n=6) at 4, 7, and 10 days after the optic nerve injury. After 2 weeks, the retrograde labeling of the RGCs was done by the dextran tetramethylrhodamine. Twenty-four hours after the labeling, the retina was wholly mounted and the labeled RGCs were counted under the fluorescence microscope.RESULTS:
The death of RGCs in this model began at 1 week and continued for 3 weeks. The number of labeled RGCs in CNTF-injected group (510+/-139/mm2) were significantly higher than that in control group (345+/-87/mm2)(p<0.05).CONCLUSIONS:
The optic nerve crush injury model was established by use of aneurysm clip. In this model, the intravitreally injected CNTF had a neuroprotective effect on the rat RGCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Optic Nerve
/
Retina
/
Retinal Ganglion Cells
/
Retinaldehyde
/
Dextrans
/
Rats, Sprague-Dawley
/
Neuroprotective Agents
/
Optic Nerve Injuries
/
Ciliary Neurotrophic Factor
/
Fluorescence
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2003
Type:
Article
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