Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia
The Korean Journal of Parasitology
;
: 195-197, 2011.
Article
in English
| WPRIM
| ID: wpr-207824
ABSTRACT
We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Taeniasis
/
Cambodia
/
Helminth Proteins
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
DNA Primers
/
DNA, Helminth
/
Taenia saginata
/
Taenia solium
/
Cyclooxygenase 1
Limits:
Animals
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2011
Type:
Article
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