Species Identification of Enterococcus by Ribotyping / 감염
Korean Journal of Infectious Diseases
;
: 377-385, 1997.
Article
in Korean
| WPRIM
| ID: wpr-208321
ABSTRACT
BACKGROUND:
Emerging drug resistance and increasing incidence of enterococci infection necessitates an accurate species identification.METHOD:
We compared the identification of twelve strains of American Type Culture Coliection(ATCC) (E. faecalis, E. avium, E. faecium, E. raffinosus, E. durans, E. casseliflavus, E. hirae, E. flavescens, E. malodoratus, E. gallnarum, E. mundtii, and E. sulfureus) and 73 clinical isolates of enterococci(E. faecalis 8, E. faecium 8, E. avium 13, E. durans 5, and unidentified strains 39) by Vitek Gram-positive Identification card (version R08.1, bio- Merieux Vitek, Hazelwood, Mo., U.S.A.), ribotyping and conventional scheme of Facklam and Sahm.RESULTS:
All ATCC strains could be identified on the basis of characteristic 16S-23S rDNA fingerprint patterns by using Eel I and Hind III. Of 39 strains unidentified by Vitek GPI card, 25 strains were identified as E. faecium, 5 strains were E. gallinarum, 5 strains were E. casseliflavus, 3 strains were E. avium and 1 strain was E. hirae.CONCLUSION:
By ribotyping with Eel I and Hind m digestion, all twelve ATCC strains and 73 clinical strains were identified and it seems to be a reliable method for identification of Enterococcus species.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Ribosomal
/
Drug Resistance
/
Incidence
/
Enterococcus
/
Ribotyping
/
Dermatoglyphics
/
Digestion
/
Eels
Type of study:
Diagnostic study
/
Incidence study
/
Prognostic study
Language:
Korean
Journal:
Korean Journal of Infectious Diseases
Year:
1997
Type:
Article
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