Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells / 한국영양학회지
Journal of Nutrition and Health
; : 167-175, 2014.
Article
in Ko
| WPRIM
| ID: wpr-20876
Responsible library:
WPRO
ABSTRACT
PURPOSE: Previous studies have shown that treatment with Smilax china L. leaf extract (SCLE) produces antidiabetic effects due to alpha-glucosidase inhibition. In this study, we examined the mechanism underlying these antidiabetic effects by examining glucose uptake in HepG2 cells cultured with SCLE. METHODS: Glucose uptake and glucokinase activity were examined using an assay kit. Expression of glucose transporter (GLUT)-2, GLUT-4, and HNF-1alpha was measured by RT-PCR or western blot. RESULTS: Treatment with SCLE resulted in enhanced glucose uptake in HepG2 cells, and this effect was especially pronounced when cells were cultured in an insulin-free medium. SCLE induced an increase in expression of GLUT-2 but not GLUT-4. The increase in the levels of HNF-1alpha, a GLUT-2 transcription factor, in total protein extract and nuclear fraction suggest that the effects of SCLE may occur at the level of GLUT-2 transcription. In addition, by measuring the change in glucokinase activity following SCLE treatment, we confirmed that SCLE stimulates glucose utilization by direct activation of this enzyme. CONCLUSION: These results demonstrate that the potential antidiabetic activity of SCLE is due at least in part to stimulation of glucose uptake and an increase in glucokinase activity, and that SCLE-stimulated glucose uptake is mediated through enhancement of GLUT-2 expression by inducing expression of its transcription factor, HNF-1alpha.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Transcription Factors
/
China
/
Blotting, Western
/
Smilax
/
Absorption
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Glucose Transport Proteins, Facilitative
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Hepatocyte Nuclear Factor 1-alpha
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Alpha-Glucosidases
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Hep G2 Cells
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Glucokinase
Country/Region as subject:
Asia
Language:
Ko
Journal:
Journal of Nutrition and Health
Year:
2014
Type:
Article