Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells / 한국영양학회지
Journal of Nutrition and Health
;
: 167-175, 2014.
Article
in Korean
| WPRIM
| ID: wpr-20876
ABSTRACT
PURPOSE:
Previous studies have shown that treatment with Smilax china L. leaf extract (SCLE) produces antidiabetic effects due to alpha-glucosidase inhibition. In this study, we examined the mechanism underlying these antidiabetic effects by examining glucose uptake in HepG2 cells cultured with SCLE.METHODS:
Glucose uptake and glucokinase activity were examined using an assay kit. Expression of glucose transporter (GLUT)-2, GLUT-4, and HNF-1alpha was measured by RT-PCR or western blot.RESULTS:
Treatment with SCLE resulted in enhanced glucose uptake in HepG2 cells, and this effect was especially pronounced when cells were cultured in an insulin-free medium. SCLE induced an increase in expression of GLUT-2 but not GLUT-4. The increase in the levels of HNF-1alpha, a GLUT-2 transcription factor, in total protein extract and nuclear fraction suggest that the effects of SCLE may occur at the level of GLUT-2 transcription. In addition, by measuring the change in glucokinase activity following SCLE treatment, we confirmed that SCLE stimulates glucose utilization by direct activation of this enzyme.CONCLUSION:
These results demonstrate that the potential antidiabetic activity of SCLE is due at least in part to stimulation of glucose uptake and an increase in glucokinase activity, and that SCLE-stimulated glucose uptake is mediated through enhancement of GLUT-2 expression by inducing expression of its transcription factor, HNF-1alpha.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
China
/
Blotting, Western
/
Smilax
/
Absorption
/
Glucose Transport Proteins, Facilitative
/
Hepatocyte Nuclear Factor 1-alpha
/
Alpha-Glucosidases
/
Hep G2 Cells
/
Glucokinase
Country/Region as subject:
Asia
Language:
Korean
Journal:
Journal of Nutrition and Health
Year:
2014
Type:
Article
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