Histologic Study after 5-Fluorouracil Injection into the Bleb in Ahmed Valve Implanted Rabbit Eyes
Journal of the Korean Ophthalmological Society
;
: 2144-2452, 2003.
Article
in Korean
| WPRIM
| ID: wpr-208763
ABSTRACT
PURPOSE:
To study the effect of the 5-fluorouracil (5-FU) injection into the bleb after the Ahmed glaucoma valve implant in rabbits.METHODS:
Ahmed valve was implanted in normal 10 rabbits without previous ophthalmic history. In 4 of 10 rabbits, 5-FU (50 mg/ml, 0.1ml) was injected into filtering bleb in every two weeks for two times at postoperative 1 month and others were followed up as a control group. We obtained histological findings in 2 rabbis of control group at postoperative 1 month. In the control group and 5-FU injected 4 rabbit eyes, each 2 rabbit eyes were enucleated at postoperative 3 months and 6 months in both groups. The dissected bleb was examined through light microscopy with hematoxyline and eosin (H and E) stain and Masson-Trichrome (M-T) stain, and electron microscopy.RESULTS:
The bleb was relatively avascular on gross and histological examination in the 5-FU injected group, and collagen fibers of the 5-FU injected group were less compact than that of the control group at postopeative 3 months. The number of inflammatory cells, fibroblasts and capillaris in outer layer was decreased in the 5-FU injected group. Under the electron microscopic examination, the density of collagen fibers was decreased and the space among them was widened. This change was more prominent in the 5-FU injected group.CONCLUSIONS:
We found that the histological changes of blebs were made by the influx of aqueous humor through the Ahmed glaucoma valve and more stimulated by injection of 5-FU into the bleb after implantation of Ahmed glaucoma valve in a rabbit model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aqueous Humor
/
Microscopy, Electron
/
Glaucoma
/
Blister
/
Collagen
/
Eosine Yellowish-(YS)
/
Fibroblasts
/
Fluorouracil
/
Hematoxylin
/
Microscopy
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2003
Type:
Article
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