Prevalence of the Extended-Spectrum beta-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 218-223, 2009.
Article
in English
| WPRIM
| ID: wpr-208982
ABSTRACT
BACKGROUND:
This study was performed to investigate the prevalence of qnr genes in clinical isolates of Escherichia coli from Korea that produce extended-spectrum beta-lactamases (ESBLs).METHODS:
During the period of May to June 2005, we collected clinical isolates of E. coli that were intermediate or resistant to ceftazidime and/or cefotaxime from 11 Korean hospitals. Antimicrobial susceptibility was determined by the disk diffusion and agar dilution methods. ESBL production was confirmed phenotypically by the double-disk synergy test. ESBL and qnr genes were searched for by PCR amplification, and the PCR products were then subjected to direct sequencing.RESULTS:
Double-disk synergy tests were positive in 84.3% (118/140) of ceftazidime- and/or cefotaxime-nonsusceptible E. coli isolates. The most prevalent types of ESBL in E. coli isolates were CTX-M-14 (N=41) and CTX-M-15 (N=58). Other ESBLs were also identified, including CTX-M-3 (N=7), CTX-M-9 (N=8), CTX-M-12 (N=1), CTX-M-57 (N=1), SHV-2a (N=2), SHV-12 (N=17) and TEM-52 (N=4). The qnrA1 and qnrB4 genes were identified in 4 and 7 ESBL-producing isolates, respectively.CONCLUSIONS:
CTX-M-type enzymes were the most common type of ESBL in E. coli isolates from Korea, and the qnr genes were not uncommon in ESBL-producing E. coli isolates. Dissemination of E. coli containing both ESBL and qnr genes could compromise the future usefulness of the expanded-spectrum antibiotics for the treatment of infections.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Beta-Lactamases
/
Polymerase Chain Reaction
/
Inhibitory Concentration 50
/
Escherichia coli Proteins
/
Escherichia coli
/
Disk Diffusion Antimicrobial Tests
Type of study:
Prevalence study
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Laboratory Medicine
Year:
2009
Type:
Article
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