Comparison of Rapid Antigen Test and Real-Time Reverse Transcription PCR for the Detection of Influenza B Virus / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 93-97, 2012.
Article
in Korean
| WPRIM
| ID: wpr-209297
ABSTRACT
BACKGROUND:
Rapid antigen test and real-time reverse transcription PCR (rRT-PCR) are widely used for the detection of influenza A virus. In this study, we evaluated and compared the effectiveness of a rapid antigen test, currently used for detecting influenza B virus, with the effectiveness of using rRT-PCR for the same purpose.METHODS:
Samples obtained from 92 patients during an outbreak of influenza B were assessed using the rapid antigen test (SD BIOLINE Influenza Ag; SD, Korea) and rRT-PCR (Anyplex FluA/B Real-time Detection; Seegene, Korea).RESULTS:
The sensitivity and specificity of the rapid antigen test were 69% and 100%, respectively, in detecting influenza B when compared to rRT-PCR. Twenty-nine patients (31.5%) were positive for both rapid antigen test and rRT-PCR, while 50 (54.3%) were negative for both rapid antigen test and rRT-PCR. The overall concordance rate between rapid antigen test and rRT-PCR was 85.9%. Thirteen patients (14.1%) were positive only for rRT-PCR.CONCLUSIONS:
The rapid antigen test showed high specificity and good correlation with rRT-PCR and is likely to be as useful in the detection of influenza B viruses.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Influenza A virus
/
Influenza B virus
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Reverse Transcription
/
Influenza, Human
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2012
Type:
Article
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