The study on cytotoxicity of cytokines produced by the activated human NKT cells on neuroblastoma / 소아과
Korean Journal of Pediatrics
;
: 439-445, 2006.
Article
in English
| WPRIM
| ID: wpr-210311
ABSTRACT
PURPOSE:
alpha-Galactosylceramide (alpha-GalCer)-stimulated human Valpha24 natural killer T (NKT) cells exert antitumor activity against some leukemia in a CD1d dependent and TCR-mediated manner, but could not kill CD1d-negative neuroblastoma (NB) cells. There are few reports about the direct antitumor effect of highly secreted cytokines by these cells on activation. In this study, using a cell-free supernatant (SPN) collected from plate bound hCD1d/alphaGalCer tetramers-stimulated NKT cells, we examined whether they could be helpful in the immunotherapeutic treatment of NB.METHODS:
Cells were cultured in IMDM. The cytokines produced by NKT cells were measured with Cytometric Bead Array (CBA) analysis. Cell viability was evaluated by calcein-AM fluorescence with digital image microscopy scanning (DIMSCAN). The percentage of specific apoptosis was calculated by flow cytometric detection of apoptosis using annexin V and 7-AAD.RESULTS:
The activated NKT cells secreted high levels of IL-2, INF-gamma, TNF-alpha. The SPN was significantly cytotoxic against four out of eight tested NB cell lines, through mainly apoptosis as evidenced by annexin-V staining and inhibition with the pretreatment of pancaspase blocker. This apoptosis was significantly inhibited when anti-TNF-alpha and anti-IFN-gamma neutralizing mAbs were used separately and it was completely abolished when the two mAbs were combined.CONCLUSION:
IFN-gamma and TNF-alpha produced by NKT cells could exert synergistically direct anti-tumor activity through apoptosis on some NB cell lines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia
/
Cell Line
/
Cell Survival
/
Cytokines
/
Interleukin-2
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Annexin A5
/
Natural Killer T-Cells
/
Fluorescence
Limits:
Humans
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2006
Type:
Article
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